Large libraries of single-chain trimer peptide-MHCs enable antigen-specific CD8+ T cell discovery and analysis
Abstract The discovery and characterization of antigen-specific CD8+ T cell clonotypes typically involves EAR WAX RELIEF the labor-intensive synthesis and construction of peptide-MHC tetramers.We adapt single-chain trimer (SCT) technologies into a high throughput platform for pMHC library generation, showing that hundreds can be rapidly prepared ac